Composite

Part:BBa_K5097017:Design

Designed by: Christopher Dorce   Group: iGEM24_Oneonta   (2024-09-28)


RFP with 6X His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 630
    Illegal AgeI site found at 742
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Removed the native stop codon, added an AGVG linker and 6x His tag. A double-stop codon was added at the end.


Source

E. coli DH5 alpha cells

References